Isolating Exosomes Derived From Human Natural Killer Cells For Characterization

Authors

  • Wesley Huang Eli and Edythe Broad Center for Regenerative Medicine
  • Jo Carmona San Marino High School

Keywords:

natural killer cells, isolating exosomes

Abstract

Exosomes have been an area of great interest in the field of research, as they seem to participate in many essential cellular processes. However, current methods in isolating exosomes have many limitations. The aim of this study is to present a more effective, efficient, and simple method to isolate large quantities of natural killer (NK) cell-derived exosomes using the ÄKTA start system, centered around size-exclusion chromatography, and to characterize these exosomes. Using the ÄKTA system, we obtained a chromatogram of high resolution depicting the UV absorbance of exosomes. We confirmed the identity of the exosomes by probing for markers such as CD56 and CD81 and cytotoxic proteins such as granzyme B in the exosomes, whose cytotoxic properties against cancer cells were confirmed using luciferase assays. By incubating exosomes, cancer cells, and an array of inhibitors, we also found that NK exosomes may engage in cytotoxicity via different methods such as ligand-receptor interactions.


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Author Biographies

Wesley Huang, Eli and Edythe Broad Center for Regenerative Medicine

I am a student of San Marino High School.

Jo Carmona, San Marino High School

Science teacher

Published

2018-12-31

How to Cite

Huang, W., & Carmona, J. (2018). Isolating Exosomes Derived From Human Natural Killer Cells For Characterization. Journal of Student Research. Retrieved from https://www.jofsr.org/index.php/path/article/view/471

Issue

Section

Research Articles