Yeast require an Intact Tryptophan Biosynthesis Pathway and Exogenous Tryptophan for Resistance to Sodium Dodecyl Sulfate


  • Amie Jo McClellan Bennington College
  • Laura M. Ammons Division of Natural Sciences and Mathematics Bennington College Bennington, VT 05201
  • Logan R. Bingham Division of Natural Sciences and Mathematics Bennington College Bennington, VT 05201
  • Sarah Callery Division of Natural Sciences and Mathematics Bennington College Bennington, VT 05201
  • Elizabeth Corley Division of Natural Sciences and Mathematics Bennington College Bennington, VT 05201
  • Katherine A. Crowe Division of Natural Sciences and Mathematics Bennington College Bennington, VT 05201
  • Jennifer K. Lipton Division of Natural Sciences and Mathematics Bennington College Bennington, VT 05201
  • Carlos A. Mendez Division of Natural Sciences and Mathematics Bennington College Bennington, VT 05201
  • Tessalyn Morrison Division of Natural Sciences and Mathematics Bennington College Bennington, VT 05201
  • Claudia Rallis Division of Natural Sciences and Mathematics Bennington College Bennington, VT 05201
Keywords: Saccharomyces cerevisiae, genome-wide chemical-genetic screen, SDS

Abstract


Sodium Dodecyl Sulfate, or SDS, is an anionic detergent with widespread use in industrial and household cleaning products, scientific laboratories, and personal care products such as toothpaste and shampoo.  The potential toxicity of SDS has been well-characterized in whole organism studies and its potential effects on the environment continue to be studied.  Herein, we undertake a chemical-genetic screen to explore whether low concentrations of SDS have any discernible effects at the cellular level.  Our screen of the homozygous diploid yeast deletion collection identified numerous gene deletions that confer sensitivity to SDS.  Subsequent bioinformatic and biological analyses reveal that yeast unable to synthesize  tryptophan are especially sensitive to the presence of SDS.  Interestingly, even wild-type yeast with an intact tryptophan biosynthetic pathway exhibit growth defects in the presence of SDS on media lacking tryptophan.  Altogether, we have shown that low levels of SDS, primarily through effects on tryptophan availability, impact the basic cell biology of a eukaryotic cell.

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Author Biography

Amie Jo McClellan, Bennington College
Since 2006, I have been the cell biology and genetics faculty member in the division of natural sciences and mathematics at Bennington College.
Published
02-01-2015

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How to Cite
McClellan, A. J., Ammons, L. M., Bingham, L. R., Callery, S., Corley, E., Crowe, K. A., Lipton, J. K., Mendez, C. A., Morrison, T., & Rallis, C. (2015). Yeast require an Intact Tryptophan Biosynthesis Pathway and Exogenous Tryptophan for Resistance to Sodium Dodecyl Sulfate. Journal of Student Research, 4(1), 74-82. Retrieved from https://www.jofsr.org/index.php/path/article/view/184
Section
Research Articles